Nuclear Magnetic Resonance Spectroscopy - Part 2: Biomolecular NMR

University of Georgia
City, State: 
Athens, GA
John N. Glushka and James H. Prestegard (University of Georgia)

This workshop (biomolecular NMR) is designed for participants who are familiar with the fundamental principles of NMR spectroscopy. There is a separate workshop for those prefering a more introductory approach to NMR. The application to attend the workshop has a series of questions that will allow applicants and the instructors to place participants in the appropriate workshop. Participants may register for one or both workshops [registration for both workshops requires completion of two separate applications].

Topics for the more advanced biomoleulcar NMR workshop include:

1. Selecting and applying a set of 2D experiments for primary structure of an oligosaccharide
2. Theory of spin relaxation, dipolar relaxation, NOE
3. Practical illustration of selective 1D NOE and 2D NOE experiments and data
4. Ligand binding: Transferred NOE (TRNOE) and saturation transfer difference (STD) experiments
5. Introduction to protein nmr:  water suppression methods
6. Introduction to protein nmr:  looking at an isotope enriched protein
7. An exercise in metabonomics:  STOCSY analysis of an enzyme reaction
8. Using available NMR processing software and databases